Raised Lateral Patch Clamp Array in Open-Acess Fluidic System

A novel lateral patch-clamp array with cell trapping locations raised above the substrate was developed. The fabrication method creates an open-access fluidic system by involving both microfabrication and macro-machining processes. This paper demonstrates the capability of using such an open-access fluidic system for high throughput patch clamp measurements. Seal resistances over 350M? were measured consistently. Patch clamp recording were obtained with from Kv2.1 potassium transfected CHO cells.

Figure caption: (a). Angled view of the device. Patch channels were filled with blue dye and cell manipulation channel were filled with green dye. (b). Close-up view of the patch clamp channels and cell manipulation channels. (c). Schematic of the set-up. (d)-(e). SEM of the patch aperture.